Journal: Nutrients

Article Title: Beneficial Activities of Alisma orientale Extract in a Western Diet-Induced Murine Non-Alcoholic Steatohepatitis and Related Fibrosis Model via Regulation of the Hepatic Adiponectin and Farnesoid X Receptor Pathways.

doi: 10.3390/nu14030695

Figure Lengend Snippet: Figure 5. Administration of AO extract affects adiponectin and farnesoid X receptor (FXR) activation. (a) Representative immunoblotting images and quantitative protein expressions for (b) adiponectin and (c) AMP-activated protein kinase (AMPK) in mice liver. (d) Representative immunoblotting images and quantitative protein expressions for (e) FXR, (f) osteopontin (OPN), (g) focal adhesion kinase (FAK), and (h) protein kinase B (AKT). The mRNA expressions of (i) phosphoenolpyruvate carboxykinase-cytosolic (PEPCK-c), (j) PEPCK-mitochondrial (PEPCK-m), (k) OPN, and (l) CYP7A1 in the mice liver. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. WD-treated group (n = 6 per group). Plus sign (+) and minus sign (−) indicate the presence and absence of oral administration of WD or AO extract in male C57BL/6 mice, respectively.

Article Snippet: Western diet (WD; D12079B) was obtained from Research Diets (New Brunswick, NJ, USA) Primary antibodies against FXR (72105S), adiponectin (2789S), phospho-AMPKα (2535 L), AMPKα (2532 L), phosphoFAK (3284S), FAK (3285S), phospho-Akt (4060S), and Akt (4685S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activation Assay, Western Blot, Control